From SNP information of the parents and the confounder, we can derive the haplotype information of parents and the linked haplotype information of disease genes. With linkage analysis on the sequencing information of the cells form the embryo, normal embryos can then be selected for transferring during IVF.
The incidence of ADO is reported to be 12.6%. The haplotype technology for genetic linkage analysis can prevent false negative results due to ADO.
All of 23 pairs of chromosomes will be sequenced in one reaction.
It takes only 1 ~ 2 weeks to complete a PGD test.
We have developed different panels for a series of monogenic disorders. Panels for other monogenic disorders can be easily developed using our innovative technology for customers.
NGS in PGD monogenic disorders detection, high accuracy, high speed and low cost.
 Nathan R. Treff, Ph.D., Anastasia Fedick, B.S., Xin Tao, M.S., Batsal Devkota, Ph.D., Deanne Taylor, Ph.D., and Richard T. Scott Jr., M.D.. Evaluation of targeted next-generation sequencing-based preimplantation genetic diagnosis of monogenic disease. Fertil Steril , 2013, 99(5): 1377-1384.
5 Steps to perform PGD
Advices will be provided to customers within 3 days after genetic information is supplied to our consultant
Pre-PGD can be done in 1 week for disorders on our list and 4 weeks for not
3 /IVF sampling
5 /Normal Embryo Transfer
HLA Typing + PGD for β-thalassaemia
The couple were both carriers of β-thalassaemia. They had son with severe β-thalassaemia through natural pregnancy.
The couple hoped to have a baby without β-thalassaemia disease through ART and treat the silk boy using umbilical-cord blood (UCB) of the second baby. We thus used PGD test to identify a healthy embryo with HLA type matched to the silk son.
Conclusion: HLA typing showed embryo 4 and embryo 7 matched with the sick boy. But embryo 4 carried HBB gene and was abandoned. Embryo 7 was normal and transferred.